April 11, 2022

The Value in Tissue Testing for P and K

When working with plant tissue data you can either react to the data with a nutrient application, or you can use the data to continue to develop your overall nutrient management strategy. Either way, routine monitoring of plant tissue test results is the starting point. While taking one tissue test at R2-R3 soybeans to identify a nutrient deficiency so that a foliar product can be added to the fungicide pass is common, it does not identify how long the deficiency endured. While on the other extreme, weekly tissue samples can be a pile of data to wade though. A good tissue testing monitoring program starts with a tissue test once the crop is well established, one or two while the crop is rapidly growing, one at the start of reproduction, and the final sample during pollination or grain fill.

When tracking tissue test results, weather and crop observation notes throughout the season, especially the week or two leading up to a sampling event, are critical. The main nutrient uptake routes for plants, diffusion and mass flow, require water. If water is limited in the root zone, short term nutrient deficiencies can appear until moisture improves. If the soil is saturated, nutrients that are taken up by mass flow will be diluted in the water and will have limited availability to the plants. 

Tissue testing provides an opportunity to identify and correct micronutrient deficiencies. If you can spray every week or two in a higher value crop, collecting tissue tests ahead of application will allow for management of low trending secondary and micronutrients. In commodity grains, weekly application of nutrients is not as practical, so being reactive to every change in a weekly collected tissue test is not practical. It is possible though with season long tissue testing to identify weak areas within the overall crop fertility plan and adjust the plan for future growing seasons.

For macronutrients like phosphorus and potassium, foliar applications in grain crops are often not enough to have a significant impact. The value to repeatedly collected tissue samples for management of phosphorus and potassium are the adjustments made to the timing and frequency of nutrient applications, target soil test levels, and rate of fertilizers in subsequent seasons. The goal for phosphorus and potassium to correct common issues that appear in tissue tests proactively in future years. To validate the effectiveness of the management changes, continue tissue testing after the implementation of the management changes.

March 17, 2022

Testing “Gray Area” Materials

Is it a soil? Is it a growing media? Is it a manure? Is it a compost? Is it a fertilizer?

Requests to analyze uncommon materials that cannot be completely classified as one of the above categories is becoming increasingly common. Some of these materials are industrial biproducts looking for a beneficial reuse. Some materials are novel products proposed to be used as “soil amendments”. Others are being evaluated to be a feedstock for composting or a component of a soilless or greenhouse media. To choose the correct testing methods, you need to be able answer a couple of questions about the material. What are your trying to learn about this product? How is this product intended to be used? Are there any regulatory testing requirements? If you can answer these questions, the following summarization of the different testing methods should help to select the appropriate analytical methods.

Soil testing methods for nutrients are done using different extracting solutions to estimate the fraction of the nutrients that are available or soon will be available for plant uptake to make fertilizer recommendations. These methods do not give any information about the total nutrient content and are generally intended to be used on soils that have not been heavily altered with other non-soil materials.

Growing media nutrient analysis is done through a process called a saturated media extraction. This process uses deionized water to extract the fraction of nutrients that a growing plant has immediate access to utilize. These methods are intended for materials with little or no natural soil in them and will be used to grow plants in directly.

Manure testing methods use a complete digestion to measure the total nutrient content of the material. Using the moisture content of the material, the nutrient levels can easily be converted into units of pounds per ton or pounds per 1000 gallons which is useful for calculating appropriate application rates. In addition to animal wastes, these methods can be utilized on many other materials that are being land applied for disposal.

Compost testing methods will produce very similar results to manure testing but are done using slightly different methods that are approved by the U.S. Composting Council. These methods are intended finished compost products that will be sold commercially. However, if a material is being evaluated as a potential feedstock for composting, it should also be analyzed using compost methods to allow for equal comparison to the final product.

Fertilizer testing utilizes many different methods depending on what nutrients or other components are requested. These methods are very accurate and intended to be used for products that require a Guaranteed Analysis for the sale of a product based on its nutrient content. Also, if a product is being tested as a potential liming material, it should be analyzed using fertilizer methods.

Please be aware that materials being tested as a beneficial reuse of a waste product, a liming material, or a fertilizer may fall under different state regulations and require additional testing such as heavy metals that are not included in ALGL’s routine test packages. If you have any questions regarding proper testing of a “gray area” material, contact your ALGL representative.

March 08, 2022

Soil Sampling in Advance

At ALGL we are seeing an increase in spring soil sampling. Spring soil samples have increased from 15% to 28% of annual soil samples in the past 10 years. Perceived concerns of seasonality impacting soil test data are being overshadowed by increased management flexibility.

Tradition has held soil samples to the fall sampling season. This works very well if the plan is to apply fertilizer in the spring, thus allowing time to make management plans over the winter months. More commonly the plan has been to collect the soil samples and turn the resulting data into fertilizer recommendations as fast as possible. This plan leaves no time to make key management decisions. The growing trend in our market is to separate soil sample collection, and fertilizer application, into separate seasons.

Soil test results will vary through the year. Often, nutrient levels are highest early in the crop growing season and decline through the growing season, with a recovery as nutrients begin leaving crop residues at harvest time. It is often argued that fall soil sampling shows the seasonally lowest soil test levels to ensure adequate crop fertility. A similar argument can be made for spring soil sampling in that it directly reflects what the starting point for crop fertility at the beginning of the growing season.

The greatest concern is with potassium soil test levels as it has the greatest chance of variability through the growing season. Demonstration samples collected over the last few years by ALGL shows that potassium levels vary less than ± 6-7% between spring and fall soil samples. This is often less than the cumulative sampling error between sampling events. The difference will have little to no impact in the resulting fertilizer application rates in most cases.  It is assumed that soil test potassium will be higher in the spring, this same demonstration data set shows that is often not correct.

Figure 1 - Management Cycle

True management of anything is a cyclic pattern. We often start with a soil test in the analysis phase, then make decisions on how and what to correct/improve. Followed by planning on how to implement those decisions. Then implementing the plan takes place. The real value in this model is on the next sampling cycle, taking the time to review the new data and determining if your goals of the previous plan were met, and if not, determining why. When soil samples are collected repeatedly at the same time of year, location, after the same crop, etc. to reduce the sampling variation, the changes in soil test values based on management become clearer.

When soil samples are collected in the same season as fertilizer is applied, all the management steps need to take place in 7 to 10 days during one of two busiest and stressful seasons, with no time to critically evaluate the data. The risk for mistakes, or less than ideal management decisions, increases using this short time frame. Sampling a season ahead of fertilizer application provides more time to evaluate the overall fertility management plan. For example, soil sampling in the spring provides the entire growing season to evaluate/refine the plan, make fertilizer purchases, prepare prescriptions ahead of time (yield data crop removal can still be added at harvest time), evaluate the crop through the growing season, and adjust based on crop prices, yields, and fertilizer prices. This leaves the only management step taking place during fall harvest is implementation of the plan.

When we let go of the notion that we need to collect soil samples in the fall and start looking to collect soil samples a season in advance of fertilizer application, the opportunities for increased and advanced management of soil fertility becomes possible.

March 04, 2022

Soil Sampling Depth - A Critical Piece of the Puzzle

When formulating a quality soil sampling plan, our focus is often directed toward parameters such as grid vs. zone, sample point locations, number of acres per sample, or number of cores per sample. However, a critical parameter that is often overlooked is sampling depth and the consistency of hitting the target depth every time. Cores that vary in collection depth by 1/2” – 1” or more can greatly impact the resulting data which translates straight to the bottom line when it is time to purchase and apply fertilizer inputs.

Nutrient concentration can vary greatly throughout the soil profile, especially under long term no-till conditions.Therefore reliable, consistent results can only be achieved when sampling depth is closely controlled. If you have employees or others helping in your sampling operation, make sure this topic is included and discussed in any training and instructions that you provide.

We often receive questions at the lab regarding sample collection in wet soil conditions.  Proper sampling can continue if good depth control can be achieved.  When the probe is placed into the soil, look in the top of the collection tube and ensure that the top of the soil core being collected is very near to the soil surface.  Under extremely saturated conditions the tip of the probe will not accurately cut through the profile and will push into the ground like a stake while compacting the soil around it resulting in inaccurate sampling depth.

Recommended sampling depth under varying cropping conditions are normally 4” in lawn and turf, 6” in no-till cropping and 8” in conventional tillage.  When choosing your desired sampling depth it is critical  to make your best choice for your conditions  and consistently sample at the same depth over time.  Most samples are collected every 2-4 years and the sampling depth must remain the same so that nutrient concentrations can be compared across collection dates and trends can be established.




Soil Test P

Soil Test K

















Long term no-till. LOI, Reported as Bray P-1, ammonium acetate K. Source ALGL 2021

As seen in the data table above, phosphorus, potassium and soil organic matter tend to decrease as sampling depth increases.

If you would like assistance with any of your sample collection plans, please contact our agronomy staff.

March 04, 2022

We Need Your Photos!

The ALGL customer photo calendar is becoming a tradition! This past year we celebrated the 5th issue of the calendar built by you. Over the past years it has been amazing to see how beautiful the world around us truly is though the eyes of our customers. Once again, we are reaching out to the best customers a business can ask for.

Do You have photos to share?  Please share with us pictures of those things in the life sciences that speak to you and show how amazing the world around us truly is. We want to see pictures that illustrate what fuels your passion for life sciences and customer service. When you get that picture captured, send it to news@algreatlakes.com along with your name, address, and brief note about the picture(s). Please submit your pictures in the highest resolution possible before September 15th. Then we will select our favorite pictures, then we will be letting our followers on Facebook vote on their favorite, to be on the cover of the 2023 calendar. Follow us on Facebook for voting details.

 Photo criteria 

  • Landscape oriented photos preferred, but not required.
  • Please share the highest possible resolution photo.
  • Please try to avoid company logos and easily identifiable faces.
  • No dangerous or illegal activities.


  • Photo submission deadline is September 15, 2022
  • One entry per person, however you may submit more than one photo.
  • Must be 18 years or older to enter.
  • Need not be present to win.
  • No purchase necessary.
  • Submitting a photo gives A&L Great Lakes permission to use the photo for promotional use.
  • Employees of A&L Great Lakes Laboratories, Inc. and their immediate families are not eligible for prizes, but may submit photos for consideration in the calendar.
  • Use of images in promotional items does not increase your odds of winning a prize.
  • Contest decisions and/or judgements by A&L Great Lakes Laboratories, Inc. are final.
March 04, 2022

Basics of Lab Data Files

The lab data generated by ALGL is delivered to clients in a variety of formats for the use in software. One key distinction about these data files is that it is lab data only. There are data file formats that are dedicated to the spatial representation of lab data. We at the laboratory do not generate these files. In most cases the GPS coordinates of the sample location is not shared with the lab.

The most common data files are CSV files. They are identified by their file extension, .csv and are commonly called “comma delimited” files. These are basic text files in which the data for a given sample is contained in a single line of text, and each piece of data is separated by a comma. These files can be viewed, opened, modified in spreadsheet programs like Excel and text applications like Notepad, but must be handled within specific parameters to maintain the integrity of the file structure. Often critical metadata, which is information that provides context to allow the data to be understood by the end user, is not contained in the data file. This metadata includes such information as the type of data being presented (analyte), units, and extraction method. This is especially true because not all software packages require this information to be explicitly given within a data file.  

A growing data file format is the Modus-xml file. Modus is a standardized system of defined terminology, metadata, and file structures that has grown from a need to manage and exchange agricultural testing data. The file format follows an XML structure, which is essentially a coding language. The Modus files have a standardized data structure and use a preset list of codes to identify all parameters of the sample such as lab method and units.

We often get requests for shape files. This is not a single file, but rather a set of files, that are often grouped in a ZIP file. Each data set is contained in three identically named files with different file extensions of .shp, .dbf, and .shx. Each file contains an aspect of the complete data set. These files are specific to GPS/GIS mapped data. The GPS coordinates of the sample location are required to generate these maps.

Often software companies electronically share data about a soil sample or set of soil samples before they arrive at the lab. This data can be as simple as a grower/farm/field, or very detailed, and is often determined by the software’s data flow. In most cases a unique identifier (serial number) is assigned to the sample or set of samples. That unique identifier accompanies the samples to the lab and is used to link the electronic data that was sent by the software ahead of time to the physical sample.  This can also provide more efficient data entry for the customer and the lab.

When the lab results are released, they are sent to the customer in a couple of ways. Some data is simply emailed to the customer for manual import. Some data is emailed to a server, and the server automates the uploading of the data to the software. Data can also be sent directly from our server through an automatic interface that processes the data and imports it into the software platform.

March 01, 2022

Fall Applied Manure. Will the Nitrogen Still be There?

Manure applications can be a valuable component of a nutrient management program but timing those applications to maximize the utilization of nitrogen can be challenging. Manures generally contain two forms of nitrogen, ammonium and organically bound nitrogen. Ammonium is immediately plant available and relatively immobile in the soil because it is a positively charged ion that held by the cation exchange capacity, like potassium. Organically bound nitrogen is also immobile in the soil, but it is not plant available until the organic matter is microbially decomposed, mineralizing the nitrogen.

In an ideal situation, manure applications would occur either into an actively growing crop or shortly before a crop is planted to gain the most benefit from the nitrogen. Unfortunately, our cold wet soil conditions in the spring and the types of application equipment we have available often do not allow for spring or early summer application. As a result, much of the manure in our region is land applied following harvest in the fall and early winter leaving several months for potential nitrogen loss.

The first step to maximizing the benefits of manure is to keep it on the field and in the soil profile. The most obvious potential loss of nitrogen when manure is fall applied is surface runoff. Runoff can be minimized by avoiding applications on saturated, frozen, or snow-covered ground; incorporation with tillage; or subsurface injection.

Once the manure has been incorporated into the soil profile, there is still the potential for substantial nitrogen loss to occur before the next crop can utilize it. The two potential mechanisms for loss are leaching and denitrification. Leaching has the greatest potential to occur on well drained soils during periods of heavy precipitation. However, for significant losses of nitrogen to occur due to leaching, the nitrogen needs to have been converted to nitrate prior to the precipitation. The conversion of ammonium to nitrate is a microbial process that will only occur when the microbes have adequate temperature and aeration in the soil to be active. Denitrification is the conversion of nitrate to gaseous forms that can be lost to the atmosphere. This is a microbial process that only occurs when soils are saturated, and the microbes are in an anerobic environment. Microbial activity is minimal when soil temperatures are below 50 degrees. If soil temperatures remain cold after application, the chances of significant nitrogen loss are minimized.

The winters and springs in this region are generally cold enough to keep most of the nitrogen in the soil profile. However, over the last few years this has not always been the case. The spring of 2017 was unusually warm with February and March temperatures reaching 70+ degrees. In one situation, using a soil nitrate and ammonium test, we were able to confirm the loss of nearly all the nitrogen following a fall hog manure application that was subsurface injected and treated with a nitrogen stabilizer. The grower in this situation had to supply a full rate of nitrogen fertilizer though sidedress to sustain his corn crop. The next season, following the very cold spring of 2018, the same grower in the same exact situation was able to confirm that no additional nitrogen was needed to produce his crop.

Following a relatively cold winter in 2022, nitrogen losses from fall applied manure should have been minimal so far. If you are planning to plant corn into a fall manured field, a pre-sidedress soil nitrate test is the best tool to assure that you have adequate nitrogen. For more information regarding sampling procedure and data interpretation, please see our fact sheet.
January 31, 2022

Testing Soil Carbon

In this newly emerging world of carbon credits, carbon markets, and carbon banking, the need to quantify and document soil carbon content is becoming more and more important. The challenge with this new market is that the testing methods for soil carbon have not been standardized across the industry. Carbon can be analyzed by several different methods and each method can give you different results.

At A&L Great Lakes Laboratories, soil carbon can currently be analyzed by 3 different methods; loss on ignition, combustion, and Walkley-Black oxidation.

The loss on ignition method is the quickest and cheapest method for estimating soil organic carbon. The process is fairly simple. A portion of the dried and ground soil sample is weighed, then put into an oven to burn off the soil organic matter. The sample is then weighed again, and the change in weight is equal to the organic matter content of the soil. From the organic matter content, the organic carbon content can be calculated by assuming that 58% of the organic matter is carbon.

The combustion method is a way to determine total soil carbon. The simplest explanation is that a weighed portion of the soil sample is heated to the point that both the mineral and organic fractions of the soil combust and the gases are then analyzed to determine the total carbon content. While this is a very accurate method, there is no way to determine whether the carbon originated from the organic matter in the soil, or the mineral fraction. The liming materials that are generally used consist of calcium and magnesium carbonates, and this test will detect this form of carbon also.

The Walkley-Black method determines the organic carbon content of a soil by measuring amount of carbon that is oxidized in a reaction with dichromate through a back titration. While this method is generally considered the most accurate method to determine soil organic carbon, it is also the slowest and most expensive. It also requires the handling of hazardous materials, so it is generally the least preferred method for occupational and environmental safety reasons.

As the carbon farming industry continues to evolve, the methods for analyzing carbon are likely to change as well. When comparing results from separate sampling events, be sure that you know what sampling procedure and method were used so a fair comparison can be made.

January 31, 2022

2021 Soil Test Data Summaries Are Available

The 2021 annual soil test summaries are available on the ALGL website at https://algreatlakes.com/pages/2021-soil-test-summaries. Your regional ALGL sales agronomist has access to regional trend graphs that show the change in soil test values from 1996 to 2021 for the Great Lakes Region, and individual states, that they can share for use in presentations and meetings as needed.

Soil Test Phosphorus levels 1996 - 2021

For those customers accounts that analyze more than 20 soil samples a year, soil test summaries are available for the account. Those summaries can be found on eDocs at https://docs.algreatlakes.com/login.aspx. Be sure to adjust the eDocs’s data filters to include “Summary” as the document type and the time filter to span December 31, when the reports were posted.

These same customers will also find a trend graph of these summaries over time, and a soil sampling history report that summarizes all the field that were sample in that given year. These soil sampling history reports are a great tool when routinely sampling on a 2-, 3-, or 4-year standard rotation. For example, when preparing to soil sample in 2022 on a 2-year cycle, go back to the 2020 Soil Sampling History Report for a list of fields that are due to be resampled in 2022.

December 30, 2021

Crop Removal Soil Fertility Done Right

For rented cropland, applying phosphorus and potassium fertilizer based on crop removal is common and simple, or so it seems. Theoretically if you apply as much nutrient as is removed in the harvest of grain and/or biomass, soil fertility levels should remain steady. This works well in a cash rent scenario. The soil fertility is maintained at over time for the landowner, and the tenant farming the land is not building up a soil fertility that they may not have access to in the future.

The crop removal fertilizer rate is calculated by multiplying the yield times the nutrient removed per unit of yield. Often this is done without routine soil testing to keep input cost down, further compounding potential issues. Actually, there are three main inputs into the crop removal fertility methodology, the crop removal factor, yield, and soil test values. Let’s look at how each factor can impact the results of a crop removal soil fertility plan.

Often book values are used for crop removal factors, these tend to be slightly higher than actual crop removal in many cases. The nutrient removed per unit of grain can vary quite a bit, biomass harvesting can vary even more. Most book values are identified so that 2/3 to 3/4 of all the values in the dataset fall below the single published value. In most cases using book values for crop removal factors will slightly overestimate the actual crop removal, this helps avoid a short fall in fertilizer application rates. For corn the nutrient removed per bushel often declines with increasing yields. When corn yields increase because of a larger corn kernel, the majority of that kernel is starch comprised of carbon, hydrogen, and oxygen. The germ of the kernel that contains nutrients like nitrogen, phosphorus, and potassium stays relatively the same size. This reduces the concentration of nitrogen, phosphorus, and potassium in the grain. Crop removal can also be impacted by growing conditions and variety. To improve the accuracy of the crop removal values, biomass and grain samples can be sent to the lab for analysis.

The value used for the yield input can be the source of the greatest errors. Yield is not defined in soil fertility calculations as past measured yield or future predicted yield. Measured yield is usually more accurate than predicted. Often the field average yield or an average of several past years of yield are used for this value. This assumes that the yield is stable from year to year and that the yield is uniform across the field. Often this is not the case. The picture below is a simple example of both issues. This field has a 4-year running average of 198.7 bpa corn, and a realistic yield goal of 200 bpa corn. However, in 2021 the field average yield was 235 bpa. If 200 bpa was used in the calculation, the calculated fertilizer rate could be 15% under actual average crop removal. For this same field if a uniform 200 bpa was to be used, 18% of the field would receive more fertilizer than was removed, and 52% would receive less fertilizer than was removed. Using a flat yield value would lead to excessive fertilizer inputs for the low yielding areas and put the high yielding regions at risk for lost yield potential. While arguments can be made about the accuracy of yield maps, they will identify regions of above average and below average yields. There is an application for variable rate fertilizer application even in a crop removal fertility program.

While soil tests are often seen as a way to predict or prescribe fertilizer application rates, they can also help monitor changes or the results of the fertility management. Soil testing this field after many years of flat rate crop removal revealed that the low yielding areas of the field did not require any fertilizer and the high yielding areas of the field had very low soil fertility levels. Soil fertility maps can be used in conjunction with yield maps to further define fertilizer application rates in a variable rate crop removal fertility plan.

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